P-GSE43745-3 - P-GSE43745-3

Type
growth protocol
Description
Starting cultures of Fusarium fujikuroi IMI58289 were grown for 72 hours in 100 mL of Darken media (Darken et al., 1959) in 300 mL Erlenmeyer flasks on a rotary shaker at 180 rpm at 28°C. 100 mL of ICI medium (Geissman et al., 1966) in 300 mL Erlenmeyer flasks was inoculated with 500 µL of the starting culture and incubated on a rotary shaker at 180 rpm at 28°C for 72 hours. The mycelium was harvested via Miracloth in a Büchner funnel, frozen in liquid nitrogen and lyophilized before RNA extraction.
Links
Experiment E-GEOD-43745