P-GSE40140-7 - P-GSE40140-7

Type
growth protocol
Description
Seeds were sown on plates with 0.55% of phytagel (Sigma-Aldrich, St. Louis, MO, USA) in half-strength MS medium (Duchefa Biochemie BV, Haarlem, Netherlands) containing 0.5% sucrose at pH 5.7, and kept at 4oC in the dark for 3 days to achieve uniform germination. The plates were then transferred to a growth chamber and placed vertically, and plants were grown at 22ºC under a 16-hour light (81 μmol s-1 m-2)/8-hour dark cycle. 5-day-old seedlings were transplanted onto fresh plates and the plates were placed vertically to prevent roots from growing into medium. The transplanted seedlings were grown in the growth chamber until they were 14-day-old.
Links
Experiment E-GEOD-40140