P-GSE40140-13 - P-GSE40140-13
nucleic acid extraction protocol
Total RNA was isolated from shoots or roots using TRIZOL reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacture's description using a 1:2 ratio of sample:TRIZOL reagent. Total RNA was subjected to DNase treatment using the TURBO RNA free kit (Ambion) according to the manufacture's instructions. Genomic DNA contamination and RNA integrity were tested on an Agilent 2100 Bioanalyzer.