P-MTAB-32422 - P-MTAB-32422
nucleic acid library construction protocol
Total RNA was cleaned using the Ribominus concentration module (Invitrogen) and rRNA depletion was carried out with the Dynabeads mRNA purification kit (Invitrogen). Libraries enriched with polyA mRNA suitable for sequencing by the SOLiD platform were created using the SOLiD Total RNA Seq Kit supplied by Applied Biosystems in combination with the SOLiD RNA Barcoding Kit, Module 1-16. In each instance, RNase III digested polyA RNA was used as input into library creation and 15 cycles of amplification were employed to produce the final libraries.