P-MTAB-32377 - P-MTAB-32377
To isolate neuronal progenitor cells (NPCs), forebrains of E13.5 Miz1+/+ or Miz1-delta-POZ embryos were cut in small pieces, digested with trypsin and filtered through sterile gauze. Cells were cultivated in 2:1 DMEM/F12 supplemented with 1xB27 (Life technologies), 20 ng/ul EGF (Biomol), 20 ng/ul basic FGF (Biomol), 1 ug/ml fungizone (Gibco) and Penicillin/Streptomycin (PAA). NPCs were passaged every seven days. Quart. neurospheres were grown for 30 days (three passages).
(Parameters: start time = 30, time unit = days, min temperature = 37, temperature unit = C, media = neurospheres medium)