P-GSE24178-5 - P-GSE24178-5

Type
grow
Description
CD43- resting B cells were isolated from mouse spleens by negative selection with magnetic beads (Miltenyi Biotech). Following isolation, resting B cells were stimulated with LPS (50 mcg/ml) and IL-4 (2.5 ng/ml) for 72 hrs. Resting splenic B cells were isolated from 6- to 8-week-old mice with anti-CD43 Microbeads (anti-Ly48; Miltenyi Biotech) and were cultured for 60 h with LPS (50 μg/ml; Sigma) and IL-4 (5 ng/ml; Sigma).
Links
Experiment E-GEOD-24178