P-GSE45382-2 - P-GSE45382-2

Type
specified_biomaterial_action
Description
PEC were incubated overnight in serum-free media (RPMI 1640, 2 mM glutamine, 10 mM HEPES, 100 U/ml penicillin G sodium, 100 μg/ml streptomycin sulfate and ITS supplement [1 mg/ml iron-free transferrin, 10 ng/ml linoleic acid, 0.3 ng/ml Na2Se, and 0.2 mg/ml Fe(NO3)3; Collaborative Biomedical Products, Bedford, MA] in the presence of porcine TGFb2 (5ng; R&D Systems) or PBS. Adherent cells were collected (>95% F4/80+) and placed in Trizol.
Links
Experiment E-GEOD-45382