P-GSE45123-4 - P-GSE45123-4

Type
labeling
Description
50 μl fragmented genomic DNA was used in the labeling reaction with 22.5 U terminal deoxynucleotidyl transferase (rTDT) (Promega) and 1 μL (1 mM) Biotin N6-ddATP (Enzo) under the following temperature cycle: 37°C for 90 min, 99°C for 15 min and 12°C for 5 min. Gel-shift assays were performed to confirm labeling efficiency. Subsequently, the labeled products were hybridized to Affymetrix Chips (Drosophila Genome 2.0) using standard Affymetrix protocols at the Salk Institute’s Genomic Facility Center.
Links
Experiment E-GEOD-45123