P-GSE45286-6 - P-GSE45286-6

Type
hybridization
Description
Biotin-labeled cDNA was mixed with Affymetrix hybridization buffer, placed onto Drosophila 2.0 arrays (Cat #900532), and incubated at 45º C for 18 h with 60 rpm rotation in Affymetrix Model 640 GeneChip Hybridization Oven. Following hybridization, arrays were washed and stained with streptavidin-phycoerythrin (Molecular Probes). Signal was amplified with anti-streptavidin antibody (Vector Laboratories), followed by staining with streptavidin-phycoerythrin (Molecular Probes) using the Affymetrix Model 450 Fluidics Station (Affymetrix).
Links
Experiment E-GEOD-45286