P-GSE32185-1 - P-GSE32185-1
Mitochondria were prepared from HEK293 and HeLa cells using Qproteome Mitochondria Isolation Kit (Qaigen, USA). The purity of mitochondria was confirmed by western blotting using different antibodies. RNA was isolated from mitochondria using miRCURYTM RNA Isolation Kit (Exiqon, Germany) according to manufacturer's instructions. RNA integrity was checked by Agilent 2100 Bioanalyzer (Ambion, USA).