P-GSE45105-3 - P-GSE45105-3
1. Prehybridize array for 1 hr in 200ml of 1xMES-Triton at 450C with 45 rpm rotation. 2. Incubate hybridization cocktail for 10min at 1000C then 10min at 450C. Spin at max speed for 3 min at RT. Transfer the supernatant to new tube. Spin for 3 more minutes use 200ml of resulted supernatant for hybridization. 3. Hybridize 18hr at 450C with 45 rpm rotation. 4. Use fluidics station EukGE-WS2v4 protocol (Affymetrix) for washing and staining.