P-CAGE-24526 - P-CAGE-24526
For two-weeks, on an agar-solidified MGRL medium containing 1.5 mM (control) sulfate as MgSO4 in a growth chamber controlled at 22 C and 16 h light cycles. After two weeks growth, transfer the plants to rockwool bricks (Nittobo, Tokyo, Japan) in a greenhouse controlled at 22 C. Day cycle (sunlight and 16 h of lamps per day) Irrigated twice a week with a liquid medium, keeping the ratio of nutrition at 1.5 mM sulfate/ 7 mM nitrate (control) in the plant pods. Harvest developing siliques at 10 and 11 day after flowering in the morning. Frozen by liquid nitrogen. Harvesting date is only once for each plant.