P-CAGE-24529 - P-CAGE-24529

Type
labeling
Description
Labeled target cRNA was prepared according to the GeneChip Expression Analysis Technical Manual (701025 Rev. 3) of Arabidopsis Genome ATH1 DNA array (Affymetrix, Santa Clara, CA, USA). - Double-stranded cDNA was prepared from 12 ug of total RNA. - Double strand cDNA synthesis using SuperScript Choice System (Invitrogen, Carlsbad, CA, USA) - Synthesis of biotin-labeled cRNA through in vitro transcription using BioArray HighYield RNA Transcript Kit (Enzo, New York, NY, USA) - Purification labeled cRNA with RNeasy column (Qiagen, Hilden, Germany) - Fragmentation of labeled cRNA. 94 C, 35 min in Fragmentation Buffer (Affymetrix).
Parameters
Amount of nucleic acid labeled, Amplification, Used Label
Links
Experiment E-ATMX-1