P-FPMI-103 - P-FPMI-103

Type
grow
Description
A human monocytic cell line, THP-1, were obtained from the American Type Culture (TIB-202) and was grown in RPMI-1640 media (Gibco, Invitrogen Life technologies, Burlington, ON), supplemented with 10% (v/v) heat inactivated fetal bovine serum, 2 mM L-glutamine, 1 mM sodium pyruvate (all from Invitrogen Life Technologies), 5 mM beta-mercaptometanol (Sigma). Cultures were maintained at 37oC in a humidified 5% (v/v) CO2 incubator up to a maximum of six passages.
Links
Experiment E-FPMI-5