P-FPMI-177b - P-FPMI-177b
Five biological samples of PA14(wild type) and five biological samples of PA14 metR::phoA mutant were grown to mid-log phase (OD600 =0.4 to 0.5) at 37C in liquid BM2-swarming media containing 62mM potassium phosphate buffer [pH7], 2 mM MgSO4, 10uM FeSO4, 0.4% (wt/vol) glucose, 0.1% (wt/vol) casamino acids.
Cells were harvested by centrifugation. RNA isolation, cDNA synthesis and hybridization to microarray slides as
max temperature, min temperature, start Time, stop Time