P-EMBL-172 - P-EMBL-172

Type
labeling
Description
About 10 ug of aRNA were incubated with 3 ug Random Primer (Invitrogen, Karlsruhe, Germany) in a total volume of 10 ul at 70C for 10 min and then cooled on dry-ice. Labelling was performed by ammino-allyl-coupling using the Atlas Glass Fluorescent Labelling Kit (Clontech, Palo Alto, USA) and Cy3-monofunctional reactive dye (Amersham Biosciences, Little Chalfont, England).
Parameters
Amount of nucleic acid labeled, Amplification, Label used
Links
Experiment E-EMBL-4