P-EMBL-77 - P-EMBL-77

Type
grow
Description
The murine fetal liver cell line AFT024 (a kind gift from I. R. Lemischka, Princeton University, Princeton) was maintained in DMEM (Gibco, Grand Island, N.Y., USA) supplemented with 20% FCS, 50 µM 2-Mercaptoethanol (Bio-Rad, Hercules, CA) as described before. Cells were grown to confluency in 24- or 96-well plates precoated with 0.1% gelatin (Specialty Media, Lavette, NJ) and maintained at 33C. AFT024 cells were then irradiated with 2000 rads using a irradiator 24 hours prior to using the feeder layer. Media was then changed to RPMI supplemented with 20% FCS (R20), Penicillin 1000 U/ml, Streptomycin 100 U/ml, and 50 µmol/l 2ME. Medium was supplemented with Flf3-Ligand (FL) 10 ng/ml and Thrombopoietin (Tpo) 10 ng/ml.
Parameters
max temperature, media, min temperature, start Time, stop Time
Links
Experiment E-EMBL-3