P-EMBL-112 - P-EMBL-112

Type
nucleic_acid_extraction
Description
Total RNA was purified using the Macherey & Nagel kit, DNase digested, and the RNA quality evaluated using the Agilent Bioanalyzer 2100 (Agilent Technologies Inc., CA, USA), 5 µg of total RNA was linearly amplified using the RiboAmp RNA amplification kit (Arcturus GmbH, Germany) yielding a 400-700 fold amplification of the mRNA in the samples. The amplified mRNA was re-evaluated on the Bioanalyzer 2100 and 5 µg was then subjected to indirect labeling with Cy3 and Cy5 (Amersham Biosciences) using the Atlas Glass Fluorescent labeling kit (BD Biosciences Clonetech, CA, USA).
Links
Experiment E-EMBL-2