P-CVDE-1 - P-CVDE-1

Type
hybridization
Description
Cy3 and Cy5 sample together are solved in 50 ul 1x DIG-Easy hybridisation buffer

(Roche Diagnostics, Mannheim, German), containing 10x Denhardt solution and 2ng/ul

Cot1-DNA (Invitrogen); sample was denaturated at 65 degrees C for 2min, hybridisation of

arrays were performed in Corning chambers 14h at 37°C;slides were washed twice in

1xSSC+0.1SDS and once in 0.1xSSC+0.1SDS before air pressure drying and scanning.
Links
Experiment E-CVDE-1