cbil.upenn.edu:RAD.Protocol:4716:Protocol - cbil.upenn.edu:RAD.Protocol:4716:Protocol

Type
labeling
Description
dsDNA (500ng - 1ug) was brought up to a volume of 45 ul, heated 95 degrees C for 5 minutes, then cooled on ice. Five ul Cy3 or Cy5 dCTP (Amersham) and 1 Ready-to-go DNA Labeling Bead - minus dCTP (Amersham) was added. The reaction was mixed gently and incubated for 30 minutes at 37 degrees C. The resulting sample was purified with a Min-Elute Cleanup Column (Qiagen) following the manufacturer's protocol.
Links
All experiments using protocol cbil.upenn.edu:RAD.Protocol:4716:Protocol: (E-CBIL-36, E-CBIL-38)