cbil.upenn.edu:RAD.Protocol:403:Protocol - cbil.upenn.edu:RAD.Protocol:403:Protocol

Type
compound
Description
Cells were grown in complete medium for 48 h, to 80% confluence, with a change of medium after 24 h. One day prior to stimulation, cells were switched to a basal medium (EBM-2) supplemented with 2% calf serum and 0.1% gentamicin sulfate amphotercin-B (starvation medium) to suppress cell cycle-specific gene expression. Cells were then stimulated for 2 h at 37C with 10 ng/ml recombinant human TNF alpha (+TNF alpha) (R & D Systems, Minneapolis, MN).
Links
Experiment E-CBIL-3