cbil.upenn.edu:RAD.Protocol:263:Protocol - cbil.upenn.edu:RAD.Protocol:263:Protocol

Type
nucleic acid hybridization to array protocol
Description
The fragmented cRNA is mixed with the hybridization cocktail, and heat shocked at 99C for 5 minutes. In the meanwhile, the probe array is filled with hybridization buffer and incubated at 45C for 10 minutes with rotation. The heated hybridization cocktail is then transferred to a 45C heat block for 5 minutes, and spun at maximum speed in a microcentrifuge for 5 minutes. Fill the probe array cartridge with the clarified hybridization cocktail and place probe array in rotisserie box in 45C oven. Hybridize for 16 hours.
Parameters
Fluidics_First Stain Time (seconds), Fluidics_Holding Temperature (C), Fluidics_Mixes per Wash A1 Cycle, Fluidics_Mixes per Wash A2 Cycle, Fluidics_Mixes per Wash A3 Cycle, Fluidics_Mixes per Wash B Cycle, Fluidics_Module, Fluidics_Number of Wash A1 Cycles, Fluidics_Number of Wash A2 Cycles, Fluidics_Number of Wash A3 Cycles, Fluidics_Number of Wash B Cycles, Fluidics_Protocol, Fluidics_Second Stain Time (seconds), Fluidics_Stain Temperature (C), Fluidics_Station, Fluidics_Third Stain Time (seconds), Fluidics_Wash A1 Temperature (C), Fluidics_Wash A2 Temperature (C), Fluidics_Wash A3 Temperature (C), Fluidics_Wash B Temperature (C)
Hardware
Affymetrix Fluidics Station 400
Links
All 13 experiments using protocol cbil.upenn.edu:RAD.Protocol:263:Protocol