cbil.upenn.edu:RAD.Protocol:404:Protocol - cbil.upenn.edu:RAD.Protocol:404:Protocol

Type
nucleic_acid_extraction
Description
Cells were lysed in a buffer containing guanidine isothiocyanate and beta-Mercaptoethanol (0.143 M). The lysate was homogenized and precipitated with 70% ethanol, transferred to a silica membrane column, and DNA and proteins were removed by a series of washes and centrifugations. Highly purified total RNA was then eluted from the column using RNase-free water.
Links
All experiments using protocol cbil.upenn.edu:RAD.Protocol:404:Protocol: (E-CBIL-17, E-CBIL-3, E-CBIL-41)