cbil.upenn.edu:RAD.Protocol:406:Protocol - cbil.upenn.edu:RAD.Protocol:406:Protocol

Type
labeling
Description
HAEC (total or amplified) RNA was denatured at 65C for 5 min and then incubated at 42C for 1 h with first strand buffer (50 mM Tris-HCl, pH 8.3, 75 mM KCl, 3 mM MgCl2), 10 mM DTT, 50 uCi 33P-dATP (PerkinElmer Life Sciences, Inc., Boston, MA), 0.5 mM dCTP, 0.5 mM dGTP, 0.5 mM dTTP, primers, and 200 U Superscript II Reverse Transcriptase. Free nucleotides, primers and enzyme were removed from labeled cDNA probes by QIAquick PCR Purification Kit (Qiagen Inc., Valencia, CA).
Parameters
primer amount, primer type
Links
All experiments using protocol cbil.upenn.edu:RAD.Protocol:406:Protocol: (E-CBIL-17, E-CBIL-3)