cbil.upenn.edu:RAD.Protocol:103:Protocol - cbil.upenn.edu:RAD.Protocol:103:Protocol

Type
hybridization
Description
Slides are pre-hybridized in 5X-SSC, 0.1% SDS, 1% BSA at 42 C for 45 min. Rinsed 5X in deionized H20 and 1X in isopropanol and dried by centrifugation for 1 min at 1000 rpm. The cDNA hybridization mix was combined with mouse Cot-1 DNA and Oligo dT buffers and incubated overnight at 42 C in a Corning hybridization chamber. Coverslip removed in 2X SSC, 0.1% SDS at room temp. Slide was washed once in 0.2X SSC, 0.1% SDS (42 C, 5 min, with agitation), once in 0.2X SSC (room temperature, 5 min, with agitation) and dried by centrifugation at 1000 rpm for 3 min.
Parameters
Mouse Cot-1 DNA Amount, Oligo dT(21) Amount
Links
All experiments using protocol cbil.upenn.edu:RAD.Protocol:103:Protocol: (E-CBIL-16, E-CBIL-34, E-CBIL-35, E-CBIL-36, E-CBIL-38, E-CBIL-39, E-CBIL-40, E-CBIL-5)