cbil.upenn.edu:RAD.Protocol:2347:Protocol - cbil.upenn.edu:RAD.Protocol:2347:Protocol
Purified, labeled cDNA was resuspended in 11 B5l of 3C SSC containing 0.75mg/ml polydA (Amersham ref: 27-4110-01), 25mM hepes and 0.23 % SDS. Before hybridization, the solution was boiled for 2 min and then allowed to cool to room temperature. The solution was applied to the microarray under a cover slip, and the slide was placed in a custom hybridization chamber which was subsequently incubated for 12 to 16 hours in a water bath at 63B0C. Before scanning, slides were washed in 0.6C SSC, 0.02% SDS for 1 min, and then 0.05C SSC for 1 min. Slides were dried before scanning by centrifugation at 500 rpm in a Beckman CS-6R centrifuge.