cbil.upenn.edu:RAD.Protocol:784:Protocol - cbil.upenn.edu:RAD.Protocol:784:Protocol

Type
hybridization
Description
Slides are pre-hybridized in 5X SSC, 0.1% SDS, 1% BSA at 42C for 45 min. Rinsed 5X in deionized H20 and 1X in isopropanol and dried by centrifugation for 1 min at 1000 rpm. The cDNA hybridization mix was combined with mouse Cot-1 DNA and oligo dT buffers and incubated overnight at 42C in a Corning hybridization chamber. Coverslip removed in 2X SSC, 0.1% SDS at room temp. Slide was washed once in 0.2X SSC, 0.1% SDS (42C, 5 min, with agitation), once in 0.2X SSC (room temperature, 5 min, with agitation) and dried by centrifugation at 1000 rpm for 3 min.
Parameters
Mouse Cot-1 DNA Amount, Oligo dT(21) Amount, T7-oligo-dT Amount
Links
All experiments using protocol cbil.upenn.edu:RAD.Protocol:784:Protocol: (E-CBIL-13, E-CBIL-37, E-CBIL-6, E-CBIL-7)