cbil.upenn.edu:RAD.Protocol:9:Protocol - cbil.upenn.edu:RAD.Protocol:9:Protocol
19 ul of hybridization buffer + blocker added to cDNA/dendrimer mix, incubated at 45C for 15 min. This mix was added to a prehybridized microarray, covered with a glass coverslip, incubated in a Corning hybridization chamber containing 10 ul of water in each reservoir overnight at 45C. Post hybridization wash followed: 10 min, once at 55C in 2X SSC, 0.2% SDS, once in 2X SSC at room temperature, once in 0.2X SSC at room temperature. Slides dried through centrifugation (1,000 rpm for 3 min).
blocker, cDNA/dendrimer mix, hybridization buffer, prehybridization buffer, prehybridization description, time between prehyb. and hyb.