P-TABM-460 - P-TABM-460

Type
grow
Description
Sterile/in vitro growth conditions: 16/8 hrs (d/n), lighting with a mix of warm and cool white light, 3 x 56 W cool white (Fluorescent tube: 840, Radium), 2 x 40 W warm light (Fluorescent tube: 830, Radium), ratio is 168/80=2,1 ;Light intensity: 100 uEm-2h-1 PAR (measured at the leaf surface) ;Other parameters: temperature: 20C - 22C; relative humidity: 65% - 70% ; medium: 1/2 x MS salts (micro and macro elements) ; Sucrose: 10 gr/l sucrose ; Buffer: 0.5 gr/l MES, pH 6.0 ; Agar: 1.0 gr/l plant tissue culture agar (brand: Lab M) ; Amount per plate: 100 ml (thickness layer: 5.5 mm) ; Plates: Falcon Integrid 150 x 25 mm round dishes ; Seeds per plate: 25 ; # of cm2 per seed: 7,25 cm2 ;Plate seal:Urgopore tape ;The number of seeds per plate depends on the growth stage to be harvested: 1.00 or earlier: 125 seeds; 1.02 : 60 seeds; 1.04: 30 seeds; 1.06 and later: 25 seeds. NB: Seeds are ‘imbibed’ (4 days, 4 C) before sowing, to improve homogeneity of germination.
Links
Experiment E-CAGE-198