P-MEXP-1017 - P-MEXP-1017

Type
nucleic_acid_extraction
Description
The frozen plant tissue (~100 mg) was ground in tiny mortars dipped in liquid nitrogen. The frozen powder was carefully transfered into 1.5 ml microcentrifuge tubes using small funnels and rubber spatulas also cooled with liquid nitrogen. Total RNA was extracted using TRIzol reagent (Invitrogen, 15596-026)according to manufacturer's instructions and purified with an RNeasy mini kit (Qiagen, 74104). 5 ug of total RNA was amplified via the aRNA MessageAmp kit (Ambion, 1750):
Parameters
Amplification, Extracted product
Links
All experiments using protocol P-MEXP-1017: (E-CAGE-1, E-CAGE-3)