base.cbu.uib.no:PROT:Protocol-75 - base.cbu.uib.no:PROT:Protocol-75

Type
nucleic_acid_extraction
Description
Total RNA was extracted in three steps 1. Modefied Phenol chloroform extraction (TRIZOL, Invitrogen, USA) were The upper aqueous phase was precipitated in 25% isopropanol and 25% RNA precipitation solution described by Chomczynski and Mackey 1995 (Biotechniques 19:942-945). 2. Genomic DNA was removed by DNase treatment using DNA-free (Ambion, Austin TX, USA). RNA was then precipitated over night in 75% ethanol and 2.5% 3M pH 5.2 NaAc. 3. An additionally purification step using RNeasy MiniElute Cleanup Kit (Qiagen, Hilden, Germany) was implemented
Links
Experiment E-BASE-11