base.cbu.uib.no:PROT:Protocol-81 - base.cbu.uib.no:PROT:Protocol-81
The hybridization solution consists of 55 ?l of 2 x GE x Hybridization buffer HI-RPM (Agilent Technologies, Palo Alto, USA), 11 ?l of 10 x Blocking Agent (Agilent Technologies, Palo Alto, USA) and 44 ?l labeled cDNA. CodStress microarray slides are pre-hybridized at 65?C for one hour in 3.5 x SSC, 0.1% SDS and 10 mg/ml BSA in a 50 ml falcon tube. Samples/hybridization solutions are added to Hybridization Gasket Slides x 4 (cat G2534-6001 Agilent Technologies, Palo Alto, USA) Hybridization are performed at 60 ?C for 16 h. Following hybridization the slides were dipped in 2 x SSC and 0.1%SDS at 65 ?C to remove the gasket slide, then washed in 1 x SSC at 65 ?C for 5 min, 0.2 x SSC for 5 min. and 0.05 x SSC at room temperature for 45 seconds subsequently before drying by centrifugation.