P-CAGE-25085 - P-CAGE-25085
-RNA extraction with RNeasy Plant Total RNA Kit (Qiagen, Hilden, Germany)
-Polysaccharide removal by ethanol precipitation.
One-tenth volume of 3 M sodium acetate (pH 5.2) was added to the RNA solution, then incubated on ice for 1hr. Precipitates were removed by centrifugation, 2.5 volume of ethanol was added, then RNA was recollected by a standard procedure of ethanol precipitation (Sambrook et al., 1989).
Amplification, Extracted product