P-CAGE-25084 - P-CAGE-25084
-Maintenance of Arabidopsis thaliana T87 cells and transgenic T87 cells
-Subculturing every 14 days (1:15 dilution) after filtration through 500 µm stainless sieve in mJPL3 medium
-Growth medium (mJPL3 medium)
-JPL macronutrient, 1/3-strength of MS micronutrient and vitamins, 0.1 g l-1 casamino acids, 15 g l-1 sucrose, 1 µM NAA, 2.5 mM 2-(N-morpholino) ethanesulfonic acid (MES), pH 5.8
-Rotational shaking (120 rpm) at 22˚C under continuous light (80 µE/s/m2)
-Cells were collected on the day 14th after subculturing.
day humidity, day temperature, light hours, light intensity, light source, media, night humidity, night temperature