P-CAGE-25084 - P-CAGE-25084

Type
grow
Description
-Maintenance of Arabidopsis thaliana T87 cells and transgenic T87 cells

-Subculturing every 14 days (1:15 dilution) after filtration through 500 µm stainless sieve in mJPL3 medium

-Growth medium (mJPL3 medium)

-JPL macronutrient, 1/3-strength of MS micronutrient and vitamins, 0.1 g l-1 casamino acids, 15 g l-1 sucrose, 1 µM NAA, 2.5 mM 2-(N-morpholino) ethanesulfonic acid (MES), pH 5.8

-Growth conditions

-Rotational shaking (120 rpm) at 22˚C under continuous light (80 µE/s/m2)

-Sampling

-Cells were collected on the day 14th after subculturing.

Parameters
day humidity, day temperature, light hours, light intensity, light source, media, night humidity, night temperature
Links
Experiment E-ATMX-8