P-CAGE-25013 - P-CAGE-25013

Type
bioassay_data_transformation
Description
The average fluorescence intensity for each fluor and for each gene was determined using the ImaGene program (BioDiscovery Inc., Los Angeles, CA). Median background fluorescence around each gene spot was calculated and subtracted from each spot. Signal values <500 (two to three times the average background stdev) were manually raised to 500 to avoid extreme expression ratios. Subgrid Loess normalization was performed using the limma library of the R statistical software (Yang YH, Dutoit S, Luu P, Lin DM, Peng V, Ngai J, Speed T: Normalization for cDNA microaaray data: a robust composite method addressing single and multiple slide systematic variation. Nucleic Acid Res. 2002, 30(4):e15).
Links
All experiments using protocol P-CAGE-25013: (E-ATMX-36, E-ATMX-37, E-ATMX-9)