P-CAGE-24583 - P-CAGE-24583
Total RNAs were isolated from the rosette leaves of individual plants using the Concert™ Plant RNA Purification Reagent (Invitrogen) and purified using the RNeasy Mini Kit (Qiagen). Concentration of total RNA was measured by spectrophotometer (Hitachi, Japan).Ten micrograms of total RNA was converted to the first strand DNA using the SuperScript II reverse transcriptase (Invitrogen) with a T7-oligo (dT)24 primer containing T7 RNA polymerase promoter sequence (GenoTech, Korea). Then, double-stranded cDNA was synthesized using SuperScript Choice system.
Amplification, Extracted product
|All experiments using protocol P-CAGE-24583: (E-ATMX-2, E-ATMX-30)|