P-CAGE-24583 - P-CAGE-24583

Type
nucleic_acid_extraction
Description
Total RNAs were isolated from the rosette leaves of individual plants using the Concertâ„¢ Plant RNA Purification Reagent (Invitrogen) and purified using the RNeasy Mini Kit (Qiagen). Concentration of total RNA was measured by spectrophotometer (Hitachi, Japan).Ten micrograms of total RNA was converted to the first strand DNA using the SuperScript II reverse transcriptase (Invitrogen) with a T7-oligo (dT)24 primer containing T7 RNA polymerase promoter sequence (GenoTech, Korea). Then, double-stranded cDNA was synthesized using SuperScript Choice system.
Parameters
Amplification, Extracted product
Links
All experiments using protocol P-CAGE-24583: (E-ATMX-2, E-ATMX-30)