P-CAGE-26287 - P-CAGE-26287

Type
nucleic_acid_extraction
Description
Each RNA sample comes from seedlings of Arabidopsis collected from two independent plates. The frozen plant tissue (~100 mg) was ground in tiny mortars dipped in liquid nitrogen. The frozen powder was transferred into 2 ml microcentrifuge tubes using spatulas cooled with liquid nitrogen. Total RNA was extracted using RNeasy Plant Mini Kit (Qiagen, Cat. No. 74904) according to manufacturer's instructions. 1 ug of total RNA was amplified via the aRNA MessageAmp II kit (Ambion, 1751), by the Genomics Facility at Centro Nacional de Biotecnologia, CSIC (Madrid, SPAIN)
Parameters
Amplification, Extracted product
Links
Experiment E-ATMX-29