P-CAGE-26455 - P-CAGE-26455
This protocol was performed by the Genomics Facility at Centro Nacional de Biotecnologia, CSIC (Madrid, SPAIN).
7.5 ug of aminoallyl-labeled amplified RNA were resuspended in 0.1 M Na2CO3 (pH 9.0) and labeled with Cy5 Mono NHS Ester (CyTMDye Post-labeling Reactive Dye Pack, Amersham). Samples were purified following the manufacturer's instructions for Megaclear TM (Ambion) and Cy5 incorporation was measured using 1 ul of the probe in the Nanodrop spectrophotometer.
Amount of nucleic acid labeled, Amplification, Used Label