Comment[ArrayExpressAccession]	E-TABM-910		
Investigation Title	Distance Effect		
Comment[Submitted Name]	Distance Effect		
Comment[AEExperimentType]	transcription profiling by array		
Comment[AEExperimentDisplayName]	Transcription profiling by array of honey bee brains to identify distance-responsive genes		
Experimental Design	"innate_behavior_design, organism_part_comparison_design, stimulus_or_stress_design, dye_swap_design, loop_design, replicate_design, co-expression_design"		
Experimental Factor Name	ORGANISMPART	ENVIRONMENTALHISTORY	INDIVIDUAL
Experimental Factor Type	organism_part	environmental_history	individual
Person Last Name	Sarma		
Person First Name	Moushumi Sen		
Person Email	moushumi@life.uiuc.edu		
Person Address	"505, South Goodwin Avenue, MH 432, Urbana, IL 61801"		
Person Affiliation	"Dept. of Entomology, University of Illinois at Urbana Champaign"		
Person Roles	submitter		
Public Release Date	2011-03-05		
Comment[ArrayExpressSubmissionDate]	2010-01-28		
Pubmed ID	20618450					
Publication Author List	"Sen Sarma M, Rodriguez-Zas SL, Gernat T, Nguyen T, Newman T, Robinson GE."					
Publication Title	Distance-responsive genes found in dancing honey bees.					
Experiment Description	"Here we show that regions of the honeybee brain involved in visual processing and learning and memory show a genomic response to distance information. Using a method that separates effects of perceived distance from effects of actual distance flown, we found that individuals forced to shift from a short to a perceived long distance to reach a feeding site showed differences in gene expression in the optic lobes and mushroom bodies relative to individuals that continued to perceive flying a short distance. "					
Protocol Name	P-TABM-4708	P-TABM-4712	P-TABM-4709	P-TABM-2966	P-MEXP-1269	P-TABM-3918
Protocol Type	grow	specified_biomaterial_action	labeling	hybridization	image_acquisition	nucleic_acid_extraction
Protocol Description	One-day-old focal bees (paint-marked on the abdomen) derived from two unrelated instrumentally inseminated queens were introduced into a colony headed by a naturally mated queen with ca. 6000 adult bees housed in a glass-walled observation hive with two frames of honeycombs. The hive was located in a climate-controlled indoor flight cage. 	"Individually marked foragers of similar ages (ca. 40 days) were trained for one day to collect 60% w/v sucrose solution by flying through a 1.83 m long tunnel with horizontal black and white striped pattern (grating frequency 1 cm) on the walls, distance experience=Short. On Day 2, the number of trips to the feeder and the dance behaviour of each focal bee were recorded manually and on videotape, respectively. On Day 3, the walls were changed to a vertical black and white striped pattern (grating frequency 1cm) Distance experience = Long, or to the same horizontal pattern to yield two groups: S-> S (horizontal pattern to horizontal pattern), S->L (horizontal pattern to vertical pattern) bees. After all focal bees took 1-3 foraging trips with the second pattern access to the tunnel was ended. 30 minutes later, all focal bees were removed from the observation hive and placed into liquid nitrogen."	"200 ng RNA was amplified using MessageAmp II aRNA Amplification kits (Ambion, Austin, TX). 2 ug of amplified RNA was labeled with Cy3 or Cy5 dye using Kreatech labeling kits (Applied Biosystems, Salt Lake City, UT). 60pmol each of two sets of labeled probe were then hybridized to the arrays"			
Protocol Parameters						
Protocol Software						
SDRF File	E-TABM-910.sdrf.txt