Investigation Title Transcription profiling of Arabidopsis development -AtGenExpress data set Comment[Submitted Name] AtGenExpress: Expression Atlas of Arabidopsis Development Experimental Design transcription profiling by array Experimental Design Term Source REF EFO Comment[AEExperimentType] transcription profiling by array Comment[AEExperimentDisplayName] Transcription profiling by array of organism parts from different strains of Arabidopsis Comment[ArrayExpressReleaseDate] Comment[AEMIAMESCORE] 5 Comment[ArrayExpressAccession] E-TABM-17 Comment[MAGETAB TimeStamp_Version] 2010-08-12 19:54:46 Last Changed Rev: 13058 Experimental Factor Name time organism part ecotype Experimental Factor Type time organism_part ecotype Experimental Factor Term Source REF Person Last Name Schmid Vingron Scholkopf Pape Demar Lohmann Davison Weigel Henz Person First Name M M B J M U S D R Person Mid Initials U. J. T. S. Person Email weigel@weigelworld.org Person Phone Person Fax Person Address Person Affiliation Max Planck Institute for Developmental Biology Person Roles submitter Person Roles Term Source REF Quality Control Type Quality Control Term Source REF Replicate Type Replicate Term Source REF Normalization Type Normalization Term Source REF Date of Experiment Public Release Date 2005-05-01 PubMed ID 15806101 Publication DOI 10.1038/ng1543 Publication Author List Schmid M.; Davison T.S.; Henz S.R.; Pape U.J.; Demar M.; Vingron M.; Scholkopf B.; Weigel D.; Lohmann J.U. Publication Title A gene expression map of Arabidopsis thaliana development Publication Status published Publication Status Term Source REF EFO Publication Status Term Accession Number EFO_0001796 Experiment Description This experiment is the result of normalizing the data previously submitted to ArrayExpress with the accession number E-AFMX-9. Protocol Name P-TABM-42 P-TABM-51 P-TABM-52 Affymetrix:Protocol:Hybridization-EukGE-WS2v4_450 P-TABM-54 P-TABM-53 P-TABM-50 Protocol Type grow nucleic_acid_extraction labeling hybridization feature_extraction image_acquisition bioassay_data_transformation Protocol Description Plants were grown in constant humidity and temperature conditions. Total RNA was extracted and amplified during labelling process (ftp://ftp.arabidopsis.org/home/tair/Protocols/AtGenExpress_probe_s_102E57.pdf) cRNA was labelled with biotin using Affymetrix Enzo Kit, in-vitro transcription method (ftp://ftp.arabidopsis.org/home/tair/Protocols/AtGenExpress_probe_s_102E57.pdf). Title: Affymetrix EukGE-WS2v4_450 Hybridization. Description: Slides were scanned using GeneChip Scanner 3000 and GCOS software. Raw data was normalized with RMA and mean and standard deviation over the repeats were calculated. Protocol Parameters growth temperature;growth humidity; Cell margin;Outlier high;Outlier low;Percentile; scanner; Protocol Hardware Affymetrix GeneChip Scanner 3000 Protocol Software Affymetrix GCOS software Affymetrix GeneChip Operating Software Protocol Contact Protocol Term Source REF mo The MGED Ontology SDRF File E-TABM-17.sdrf.txt Term Source Name mo nasc ncbitax tair_dev:1.27 po_development_and_growth_ontology1.1 Plant Ontology tair_anat:1.14 tair_anat:1.2 po_structure_ontology1.1 tair The MGED Ontology ArrayExpress EFO mo The MGED Ontology Term Source File http://mged.sourceforge.net/ontologies/MGEDontology.php http://arabidopsis.info/catalogue.html http://www.ncbi.nlm.nih.gov/Taxonomy/taxonomyhome.html http://www.arabidopsis.org/info/ontologies/ http://www.plantontology.org/ http://www.arabidopsis.org/info/ontologies/ http://www.arabidopsis.org/info/ontologies/ http://arabidopsis.org http://mged.sourceforge.net/ontologies/MGEDontology.php http://www.ebi.ac.uk/arrayexpress http://www.ebi.ac.uk/efo/ http://mged.sourceforge.net/ontologies/MGEDontology.php http://mged.sourceforge.net/ontologies/MGEDontology.php Term Source Version 1.27 1.14 1.2