Investigation Title Transcription profiling analysis of microarray coverage of Univeral Human Reference RNA (UHRR) on 43,000-spot human microarrays with yeast controls Comment[Submitted Name] Microarray Coverage of UHRR on 43,000-spot human microarrays with yeast controls Experimental Design reference_design transcription profiling by array Experimental Design Term Source REF mo EFO Comment[ArrayExpressReleaseDate] 2005-11-01 Comment[AEMIAMESCORE] 4 Comment[ArrayExpressAccession] E-SMDB-2652 Comment[MAGETAB TimeStamp_Version] 2011-06-28 23:15:02 Last Changed Rev: 14857 Experimental Factor Name Experimental Factor Type Experimental Factor Term Source REF Person Last Name Demeter Perou Person First Name Janos Charles Person Mid Initials Person Email jdemeter@genome.stanford.edu cperou@med.unc.edu Person Phone 726-0075 919-843-5740 Person Fax 723-7016 919-966-3015 Person Address Dept. Genetics, CCSR 2255, Stanford, CA, USA, 94305 Lineberger Comprehensive Cancer Center, Department of Genetics, CB# 7295, Room 12-046, Chapel Hill, NC, USA Person Affiliation Stanford University Univ. North Carolina at Chapel Hill Person Roles submitter submitter Person Roles Term Source REF mo mo Quality Control Type Quality Control Term Source REF Replicate Type Replicate Term Source REF Normalization Type Normalization Term Source REF Date of Experiment Public Release Date 2005-11-01 PubMed ID 15113400 Publication DOI 15113400 Publication Author List Novoradovskaya N, Whitfield ML, Basehore LS, Novoradovsky A, Pesich R, Usary J, Karaca M, Wong WK, Aprelikova O, Fero M, Perou CM, Botstein D, Braman J Publication Title Universal Reference RNA as a standard for microarray experiments. Publication Status journal_article Publication Status Term Source REF Experiment Description Microarray coverage of UHRR was determined by counting the number of spots with intensities exceeding the 1% and 5% false positive rates defined using the yeast negative control spots. Seven microarrays from different print runs were used. In four experiments, the UHRR was labeled with Cy5 and in three experiments with Cy3. Labeled UHRR was co-hybridized with Common Reference RNA (CRF) developed at Stanford University (8) labeled with the alternate dye. Protocol Name Protocol Type Protocol Description Protocol Parameters Protocol Hardware Protocol Software Protocol Contact Protocol Term Source REF SDRF File E-SMDB-2652.sdrf.txt Term Source Name ncbitax mo ArrayExpress mo EFO Term Source File http://www.ncbi.nlm.nih.gov/Taxonomy/taxonomyhome.html http://mged.sourceforge.net/ontologies/MGEDontology.php http://www.ebi.ac.uk/arrayexpress http://mged.sourceforge.net/ontologies/MGEDontology.php http://www.ebi.ac.uk/efo/ Term Source Version