Comment[ArrayExpressAccession] E-MTAB-743 Investigation Title Transcription profiling by RNA-seq Comment[Submitted Name] Transcription profiling by RNA-seq Experiment Description RNA sequencing analysis was performed to determine genome-wide mRNA expression in MCF10A cells Experimental Design co-expression_design Comment[AEExperimentType] transcription profiling by high throughput sequencing Experimental Factor Name CELL_LINE Experimental Factor Type cell_line Quality Control Type Public Release Date 2012-08-01 Person Last Name Kim Person First Name Tae Hoon Person Mid Initials Person Email taehoon.kim@yale.edu Person Phone 1-203-785-7501 Person Address Department of Genetics; 333 Cedar Street, SHM I-142B; P. O. Box 208005; New Haven, CT 06520-8005 USA Person Affiliation Yale University Person Roles submitter PubMed ID Publication Author List Publication Title Publication Status Protocol Name P-MTAB-22773 P-MTAB-22774 P-MTAB-22775 Protocol Type grow nucleic_acid_extraction sequencing Protocol Description MCF10A cells were cultured with DMEM/F12 media supplemented with 2.5% Horse serum, 0.5ug/mL hydrocortisone, 10ug/mL Insulin, 20ng/mL EGF and 100ng/mL cholera toxin. MCF10A cells were lysed in Trizol reagent (Invitrogen) and total RNA was extracted according to the manufacturer's directions. Total RNA was processed by the Yale Center for Genomic Analysis for Whole Transcriptome Analysis (mRNA-seq) following the instructions from Illumina, Inc. Protocol Hardware Illumina Genome Analyzer Comment[SecondaryAccession] ERP000799 Comment[SequenceDataURI] http://www.ebi.ac.uk/ena/data/view/ERR039513 SDRF File E-MTAB-743.sdrf.txt