Comment[ArrayExpressAccession]	E-MTAB-395
Investigation Title	Mouse F2 Cross, Carbohydrate Diet, Fat Tissue																						
Comment[Submitted Name]	Mouse F2 Cross, Carbohydrate Diet, Fat Tissue
Experiment Description	Gene transcription in epididymal fat pads was investigated in an F2 cross of 129S6 x Balb/c mice using Illumina gene expression arrays. Expression data was determined in 5 months old male mice fed a carbohydrate diet. Genotyping data for the F2 offspring used in this study can be found in the file E-MTAB-395.additional.zip under the Browse all available files link (file mouse-f2-chd-genotype.txt added  8th April 2014).																						
Experimental Design	strain_or_line_design	co-expression_design																					
Comment[AEExperimentType]	transcription profiling by array																						
Comment[AEExperimentDisplayName]	Transcription profiling by array of mouse F2 cross with carbohydrate diet to investigate stress on fat tissue																						
Experimental Factor Name	strain																						
Experimental Factor Type	strain																						
Quality Control Type																							
Public Release Date	2014-01-20																						
Person Last Name	Otto																						
Person First Name	Georg																						
Person Mid Initials	W																						
Person Email	gwo@well.ox.ac.uk																						
Person Phone																							
Person Address	The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford, OX3 7BN, United Kingdom																						
Person Affiliation																							
Person Roles	submitter																						
PubMed ID																							
Publication Author List	Dominique Gauguier, Jene Manning, Karene Argoud, Marie Therese Bihoreau, Astrid Authier, Georg W. Otto																						
Publication Title																							
Publication Status																							
Protocol Name	P-MTAB-17184	P-MTAB-17185	P-MTAB-17186	P-MTAB-17187	P-MTAB-17188
Protocol Type	grow	specified_biomaterial_action	nucleic_acid_extraction	labeling	bioassay_data_transformation																		
Protocol Description	Male mice of the 129S6 and BALB/c strains were bred locally using a stock originating from the Jackson laboratory. Hybrids of the 129S6 x BALB/c F2 cross were bred locally. All mice were maintained on a 12-h light and dark cycle with light off at 8pm. Temperature was maintained at 20-22 °C. Mice were weaned at 3 weeks and housed in groups of 10 mice in standard conditions in open top cages according to UK Home Office regulations. Mice had free access to water and standard laboratory chow pellets (B&K Universal, Hull, UK) from weaning.	Carbohydrate diet, standard laboratory chow pellets (B&K Universal, Hull, UK).	RNEASY 96 Universal Tissue Kit	ILLUMINA TOTALPREP-96 RNA AMPLIFICATION KIT	normexp background correction and quantile normalization using control probes (limma, Smyth 2004)																		
Comment[AdditionalFile:TXT]	mouse-f2-chd-genotype.txt																						
SDRF File	E-MTAB-395.sdrf.txt