Comment[ArrayExpressAccession]	E-MTAB-2118
MAGE-TAB Version	1.1
Investigation Title	Gene expression profiling of Resto6 human stromal cells
Comment[Submitted Name]	Gene expression profiling of Resto6 human stromal cells
Experiment Description	Resto6 stromal cells support the long-term survival of human plasma cells differentiated in vitro. Whole genome gene expression microarrays made it possible to pick up genes whose products could be involved in a biological pathway inducing long-term survival of plasma cells.
Experimental Design	individual_genetic_characteristics_design	in_vitro_design	co-expression_design
Comment[AEExperimentType]	transcription profiling by array
Experimental Factor Name	cell type
Experimental Factor Type	cell type
Public Release Date	2013-12-11
Person Last Name	Tarte	Jourdan
Person First Name	Karin	Michel
Person Mid Initials
Person Email	karin.tarte@univ-rennes1.fr	michel.jourdan@inserm.fr
Person Phone	33 (0)2 232 345 12	33 (0)4 67 33 04 10
Person Address	Director INSERM U917-MICA. Head of the Immunology and Cell Therapy Lab at Rennes. University Hospital. Medical School of Rennes. 2, avenue du Pr Léon Bernard. 35043 RENNES	U1040, Institut de Recherche en Biothorapie Hopital Saint-Eloi, 80 Avenue Augustin Fliche, 34295 Montpellier, France
Person Affiliation	INSERM	INSERM
Person Roles	investigator	submitter
PubMed ID	16985173
Publication Author List	Patricia Amé-Thomas, Hélène Maby-El Hajjami, Céline Monvoisin, Rachel Jean, Delphine Monnier, Sylvie Caulet-Maugendre, Thierry Guillaudeux, Thierry Lamy, Thierry Fest and Karin Tarte
Publication Title	Human mesenchymal stem cells isolated from bone marrow and lymphoid organs support tumor B-cell growth: role of stromal cells in follicular lymphoma pathogenesis
Publication Status	published
Protocol Name	P-MTAB-36380	P-MTAB-36531
Protocol Type	growth protocol	normalization data transformation protocol
Protocol Description	Stromal cells were obtained from human tonsils collected from children undergoing routine tonsillectomy, after informed consent was obtained. Tonsils were cut into pieces and flushed using syringe and needle. Cell suspension was treated with DNAse I (Pulmozyme, Roche, Neuilly sur Seine, France) and collagenase IV (Worthington, Freehold, NJ) followed by centrifugation on a discontinuous Percoll gradient (Amersham, Piscataway, NJ). LN-derived stromal cells called ÒRestoÓ were established from the 15%/25% Percoll interface by long-term culturing. Cells were initially allowed to adhere for 48 hours followed by elimination of nonadherent cells and culture in RPMI 1640 (Invitrogen, Carlsbad, CA) supplemented with 10% selected fetal calf serum (FCS; HyClone, Logan, UT), and penicillin/streptomycin (PS). To study the gene expression profile, Resto-6 stromal cells were harvested at passage 5. Cell cultures were performed in RPMI1640 supplemented with 10% foetal calf serum.	Standard Affymetrix protocol
Protocol Term Source REF	EFO	EFO
Term Source Name	EFO	ArrayExpress
Term Source File	http://www.ebi.ac.uk/efo	http://www.ebi.ac.uk/arrayexpress
SDRF File	E-MTAB-2118.sdrf.txt