Comment[ArrayExpressAccession]	E-MTAB-1840
MAGE-TAB Version	1.1
Investigation Title	Glioma miRNA expression profile
Comment[Submitted Name]	Glioma miRNA expression profile
Experiment Description	Comparison of the miRNAs profile of 8 WHO grade II gliomas and 24 higher grade tumors (2 WHO grade III and 22 glioblastomas) in order to identify the molecular determinants of progression.
Experimental Design	disease_state_design	ex_vivo_design	co-expression_design
Comment[AEExperimentType]	microRNA profiling by array
Comment[AEExperimentDisplayName]	microRNA profiling by array of 8 WHO grade II gliomas and 24 higher grade tumors to identify the molecular determinants of tumor progression
Experimental Factor Name	disease	tumor grading
Experimental Factor Type	disease	tumor grading
Quality Control Type	biological replicate
Quality Control Term Source REF	EFO
Public Release Date	2014-03-15
Person Last Name	Carella
Person First Name	Massimo
Person Mid Initials
Person Email	m.carella@operapadrepio.it
Person Phone	390882416350
Person Address	viale cappuccini, San Giovanni Rotondo (FG), Italy
Person Affiliation	Medical Genetics Unit, IRCCS Casa Sollievo della Sofferenza
Person Roles	submitter
PubMed ID	25279461
Publication Author List	Barbano R, Palumbo O, Pasculli B, Galasso M, Volinia S, DAngelo V, Icolaro N, Coco M, Dimitri L, Graziano P, Copetti M, Valori VM, Maiello E, Carella M, Fazio VM, Parrella P.
Publication Title	A MiRNA Signature for Defining Aggressive Phenotype and Prognosis in Gliomas.
Publication Status
Protocol Name	P-MTAB-34381	P-MTAB-34382	P-MTAB-34383	P-MTAB-34384
Protocol Type	growth protocol	nucleic acid extraction	labeling 	normalization data transformation
Protocol Description	Glioma samples were treated by surgery at the Department of Neurosurgery of the IRCCS Casa Sollievo della Sofferenza San Giovanni Rotondo, FG (Italy). All tissue samples were snap-frozen in liquid nitrogen after resection and stored at -80 degrees C until use.	About 150-200 mg fresh frozen tissues were used to isolate total RNA by phenol extraction (TRIzol Reagent, Invitrogen Corporation, Carlsbad, CA, USA). RNAs from normal brain cells were prepared according to manufacturer's instructions.	3DNA Array Detection FlashTag(TM)  RNA Labeling Kit (http://www.genisphere.com), according to manufacturer's recommendations	microRNA chip data were normalized using Robust Multi-array Average (RMA) algorithm
Protocol Term Source REF	EFO	EFO	EFO	EFO
Term Source Name	EFO	ArrayExpress
Term Source File	http://www.ebi.ac.uk/efo	http://www.ebi.ac.uk/arrayexpress
SDRF File	E-MTAB-1840.sdrf.txt
Publication DOI	10.1371/journal.pone.0108950