Comment[ArrayExpressAccession]	E-MTAB-1743
MAGE-TAB Version	1.1
Investigation Title	WBERRABAH
Comment[Submitted Name]	WBERRABAH
Experiment Description	Effect of variation of glucose concentration in FXR target genes expression in liver
Experimental Design	co-expression_design	in_vitro_design	compound treatment design
Comment[AEExperimentType]	transcription profiling by array
Comment[AEExperimentDisplayName]	Transcription profiling by array of the effect of glucose concentration variation and GW4064 FXR agonist on HepG2 cells
Experimental Factor Name	dose_1	compound_1	dose_2	compound_2
Experimental Factor Type	dose	compound	dose	compound
Public Release Date	2013-12-12
Person Last Name	BERRABAH
Person First Name
Person Mid Initials
Person Email	wahiba.berrabah@inserm.fr
Person Phone
Person Address
Person Affiliation
Person Roles	submitter
PubMed ID
Publication Author List	Wahiba Berrabah, Pierrette Aumercier, Céline Gheeraert, Hélène Dehondt, Emmanuel Bouchaert, Jérémy Alexandre, Maheul Ploton, Claire Mazuy, Sandrine Caron, Julie Chevalier, Jérôme Eeckhoute, Tony Lefebvre, Bart Staels and Philippe Lefebvre
Publication Title	The glucose sensing O-GlcNacylation pathway regulates the nuclear bile acid receptor FXR
Publication Status	in preparation
Protocol Name	P-MTAB-33512	P-MTAB-33513	P-MTAB-33514	P-MTAB-33515	P-MTAB-33516
Protocol Type	treatment protocol	nucleic acid extraction protocol	nucleic acid labeling protocol	hybridization protocol	array scanning and feature extraction protocol
Protocol Description	HepG2 cells were treated with 1 or 25 mM of glucose and 2 uM of GW4064 or  DMSO during 24 hours. GW4064: FXR agonist.	Total RNA was isolated using RNeasy Kit according to the manufacturers protocol (Qiagen)	Microarray labelling, hybridization and scanning were carried out following the manufacturers instructions (Agilent, One-color Microarray Gene Expression Analysis) using human SurePrint 8*60K arrays	Microarray labelling, hybridization and scanning were carried out following the manufacturers instructions (Agilent, One-color Microarray Gene Expression Analysis) using human SurePrint 8*60K arrays	Microarray labelling, hybridization and scanning were carried out following the manufacturers instructions (Agilent, One-color Microarray Gene Expression Analysis) using human SurePrint 8*60K arrays
Protocol Term Source REF	EFO	EFO	EFO
Term Source Name	EFO	ArrayExpress
Term Source File	http://www.ebi.ac.uk/efo	http://www.ebi.ac.uk/arrayexpress
SDRF File	E-MTAB-1743.sdrf.txt