Comment[ArrayExpressAccession]	E-MTAB-1715
MAGE-TAB Version	1.1																																
Investigation Title	Tbrucei_H1_RNAi																																
Comment[Submitted Name]	Tbrucei_H1_RNAi
Experiment Description	Our goal was to determine if histone H1 is involved in transcription regulation. RNA interference was used to deplete histone H1 from bloodstream form T. brucei in culture. Total RNA was extracted and RNAseq was used to compare the expression profile of H1 depleted clones and parental cell-line. 																																
Experimental Design	biological variation design	cellular modification design																															
Comment[AEExperimentType]	RNA-seq of coding RNA																																
Comment[AEExperimentDisplayName]	RNA-seq of coding RNA in bloodstream form of Trypanosoma brucei with or without depletion of histone H1 by RNA intereference to study if histone H1 is involved in transcription regulation																																
Experimental Factor Name	RNA interference																																
Experimental Factor Type	RNA interference																																
Quality Control Type	biological replicate																																
Quality Control Term Source REF	EFO																																
Public Release Date	2014-07-10																																
Person Last Name	Manso																																
Person First Name	Helena																																
Person Mid Initials																																	
Person Email	hmanso@fm.ul.pt																																
Person Phone	00 351 217 999 561																																
Person Address	Avenida Porofessor Egas Moniz, Edificio Egas Moniz, 1649-028 Lisboa, Portugal																																
Person Affiliation	Avenida Porofessor Egas Moniz, Edificio Egas Moniz, 1649-028 Lisboa, Portugal																																
Person Roles	submitter																																
PubMed ID																																	
Publication Author List	Ana C. Pena, Mafalda R. Pimentel, Helena Manso, Rita Vaz-Drago, Daniel Neves, Francisco Aresta-Branco, Filipa R. Ferreira, Fabien Guegan, Luis Pedro Coelho, Maria Carmo-Fonseca, Nuno L. Barbosa-Morais, Luisa M. Figueiredo																																
Publication Title	Trypanosoma brucei histone H1 inhibits RNA Polymerase I transcription and is important for parasite fitness in vivo																																
Publication Status																																	
Protocol Name	P-MTAB-33258	P-MTAB-33259	P-MTAB-33260	P-MTAB-33261	P-MTAB-33262
Protocol Type	growth protocol	treatment protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol																												
Protocol Description	T. brucei bloodstream form parasites (strain Lister 427, antigenic type MiTat 1.2, clone 221a) [Johnson and Cross, 1979] were cultured at 37 degrees C, 5% CO2, in HMI-11 medium.	RNA interference of histone H1 was induced for 4 days in culture.	Total RNA was extracted using TRIzol standard protocol.	Ribosomal RNA was depleted with RiboMinus kit (Invitrogen). PolyA-containing RNA was purified using Dynabeads oligo-(dT) (Invitrogen) and cDNA was synthesized using random hexamers according to Complete RNA-seq Library System kit (NuGEN) instructions. 	Illumina HiSeq2000 standard protocol																												
Protocol Term Source REF	EFO	EFO	EFO	EFO	EFO																												
Protocol Hardware					Illumina HiSeq 2000																												
Term Source Name	EFO
Term Source File	http://www.ebi.ac.uk/efo																																
Comment[SecondaryAccession]	ERP003403																																
Comment[SequenceDataURI]	http://www.ebi.ac.uk/ena/data/view/ERR556936-ERR556939																																
SDRF File	E-MTAB-1715.sdrf.txt