Comment[ArrayExpressAccession]	E-MTAB-1544
Investigation Title	miR expression during chondrogenic differenciation on mesenchymal stem cells																					
Comment[Submitted Name]	miR expression during chondrogenic differenciation on mesenchymal stem cells
Experiment Description	Differentiation of mesenchymal stem cells relies on precise control of tightly controlled genetic programme where specific sets of genes are up-regulated. Identification of miRNAs that are modulated during this process should help to better understand cho																					
Experimental Design	cell_type_comparison_design	in_vitro_design	co-expression_design																			
Comment[AEExperimentType]	transcription profiling by array																					
Comment[AEExperimentDisplayName]	Transcription profiling by array of human bone marrow treated (TGFb3M) and control esenchymal stem cells to investigate miRNAs modulated during first stages of chondrogenic differentiation																					
Experimental Factor Name	time	compound	dose																			
Experimental Factor Type	time	compound	dose																			
Quality Control Type	biological_replicate																					
Public Release Date	2014-03-20																					
Person Last Name	CHUCHANA																					
Person First Name	Paul																					
Person Mid Initials																						
Person Email	paul.chuchana@inserm.fr																					
Person Phone	(33)499636100																					
Person Address	H?pital Saint-Eloi, B?t INM 80 avenue Augustin Fliche France 34295 MONTPELLIER																					
Person Affiliation	INSERM U844																					
Person Roles	submitter																					
PubMed ID																						
Publication Author List	Chuchana P., Gu?rit D. and Noel D.																					
Publication Title	Sox9-regulated miRNA-574-3p inhibits chondrogenic differenciation on mesenchymal stem cells																					
Publication Status	In revision																					
Protocol Name	P-MTAB-31877	P-MTAB-31878	P-MTAB-31879	P-MTAB-31880	P-MTAB-31881
Protocol Type	grow	nucleic_acid_extraction	Labeling	hybridization	image_acquisition																	
Protocol Description	Human Mesenchymal Stem Cell growth were cultured in alpha MEN , 10% FCS, 10% L-Glutamin with or without  TGFB3 (10ng/ml)	RNA was extracted with the RNeasy Kit (Qiagen) or the SV-total RNA extraction kit (Promega) and Trizol (Invitrogen), in accordance with the manufacturer?s instructions.	Biotinylated complementary RNA (cRNA) was amplified with a double in-vitro transcription, according to the Affymetrix small sample labelling protocol vII (Affymetrix). The biotinylated cRNA was fragmented and hybridized to the HG-U133 Plus 2.0 GeneChip ol	The samples were hybridized for 16 hr at 48?C and 60 rpm on miRNA-1_0 Array in the Affymetrix Hybridization Oven 645. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 with the Hybridization Wash & Stain kit and the Command Console 	The arrays were scanned using the Affymetrix GeneChip Scanner 3000 7G and the Command Console software.																	
SDRF File	E-MTAB-1544.sdrf.txt